Wednesday, June 5^th

4:00 – 5:00 pm Registration Table Open (Biochemistry Atrium)

5:15pm Opening Remarks (Marisa Otegui)

5:30 - 6:15 Keynote: Luis Vidali, Worcester Polytechnic Institute (Biochemistry Room 1125)

• A motor in the driver’s seat: myosin and vesicles anticipate F-actin and drive polarized plant cell growth

6:30 - 9:00 pm Poster Social. Reception with food and beverages. Cash Bar. (Biochemistry Atrium)

Put up posters in Biochemistry Atrium

Food and beverages until 9:00 pm



Thursday, June 6^th

Oral presentations: Participating labs have either a 15 or 30 min. time slot. The PI has the option to provide a very brief overview of the research (2-5 min.) and for 30 min slots the remaining time can be divided among one or two speakers. 4 minutes will be reserved for questions at the end of the presentations.

7:30 - 8:30 am Coffee and Morning Snacks. (Biochemistry Atrium)

8:30 - 9:15 am Keynote: Wendy Silk (Biochemistry Room 1125)

• Straps, hooks, and ruffles: Mechanics of curving and twisting in sessile, growing organisms

9:15 - 10:15 am Cytoskeleton (session chair: Chris Staiger) Biochemistry Room 1125

• 9:15 - 9:30 Vidali Lab
• 9:30 -10:00 Staiger Lab
• 10:00 - 10:15 Smith Lab
• 10:15 – 10:30 Szymanski Lab

10:30 to 11: 00 Coffee break (Biochemistry Atrium)

11:00- 12:15 Autophagy and protein degradation (session chair: Diane Bassham) Biochemistry Room 1125

• 11:00-11:30 am Bassham Lab
• 11:30-12:00 am Vierstra Lab
• 12:00 – 12:15 Howell Lab

12:15 to 1:30 pm lunch (on your own)

1:30 - 3:30pm Workshop I: Kinematics (organized by Wendy Silk, Edgar Spalding, and Nathan Miller) Biochemistry Building 420 Henry Mall; Rooms B1144A and B1144B

• This workshop will comprise a set of hands-on activities designed to show how the motion and behavior of biological material during growth is studied. The workshop will consist of two parts. First, Wendy Silk will explain the need for objective methods to analyze growth patterns. Emphasizing the importance of material (cell-specific) and spatial (site-specific) descriptions she will review growth analysis in terms of growth trajectories, growth velocities, and relative elemental growth rates. Participants will then practice some simple spreadsheet calculations for growth rate, relative growth rate, and growth velocity in a moving reference frame. The first section will conclude with an appreciation of pitfalls in using long-term marking experiments to infer growth patterns. Next, Nathan Miller will help participants perform kinematic analysis of root growth using a computational tool running on the iPlant cloud and existing image sets

3:30-4:00pm: Coffee Break (Biochemistry Atrium)

4:00 to 6:00 Workshop II: Fluctuation cross-correlation analysis for the study of fluorescent reporters in tip growth (organized by Luis Vidali). Computer lab Mechanical Engineering ME2109

• This workshop will present basic concepts in image and signal processing to perform fluctuation cross-correlation analysis of time series. The focus will be on analyzing the dynamics of fluorescent probes in the tip growing cells from the model plant Physcomitrella patens. The necessary ImageJ and Matlab macros will be provided and their use demonstrated. Alternative uses of this type of analysis to study other cellular processes will be discussed.

6:00 to 7:30 pm Poster Social, Food and beverages. Cash bar

7:30 to 8:30 pm Organelles and Trafficking I (session chair: Ying Gu) Biochemistry Room 1125

• Plastids and mitochondria
• • 7:30-7:45 Osteryoung Lab
  • 7:45- 8:00 Logan Lab
• Trafficking I
• 8:00-8:15 Nebenführ Lab
• 8:15-8:30 Brandizzi Lab

8:30 to 10:00pm Poster Session I Biochemistry Atrium. Food and beverages. Cash Bar

8:30 to 9:30 pm Even posters present



Friday, June 7^th

7:30 - 8:30 am Coffee and Morning Snacks

8:30- 10:00am Trafficking II (session chair: Diane Bassham) Biochemistry Room 1125

• 8:30-9:00 Bednarek Lab
• 9:00-9:30 Otegui Lab
• 9:30- 10:00 Gu Lab

10:00 to 10:30 coffee break. (Biochemistry Atrium)

10:30 to 12:15 Signaling I (session chair: Gabriele Monhausen) Biochemistry Room 1125

• 10:30-11:00 Monshausen Lab
• 11:00-11:15 Haswell Lab
• 11:15-11:45 Gilroy Lab
• 11:45-12:15 Masson Lab

12:00 to 3:00 pm Lunch Break

3:00 to 4:30 Signaling II (Session chair: Edgar Spalding) Biochemistry Room 1125

• 3:00 - 3:15 Heese Lab
• 3:15 - 3:30 Debolt Lab
• 3:30 - 4:00 Spalding Lab
• 4:00 - 4:15 Nielsen Lab

4:15 to 4:45 Coffee and beverage break

4:45 to 6:30 Signaling III (session chair: Dan Szymanski) Biochemistry Room 1125

• 5:45-5:00 Luan Lab
• 5:00-5:30 Ane Lab
• 5:30 – 5:45 Szymanski Lab

6:00 to 10:00 pm Poster Session II. (Biochemistry Atrium). Poster Social and Food and Cash Bar

8:00- 9:00 Odd numbered posters present

9:30 pm 2014 Poster Figure Image Contest: votes are due

10:00 pm Meeting ends

Sponsors of the 2013 meeting
The organizers gratefully acknowledge the support of: